The article interprets accessibility as a systemic approach that crosses all museum functions, transforming it into a social infrastructure oriented towards well-being, participation, and recognition. Through the case of the Uffizi Galleries, it is shown how cultural mediation and inclusive design can redefine the relationship with diverse audiences. The establishment of the Department of Cultural Mediation and Accessibility marks a shift towards practices based on direct experience, listening, and co-construction. The tools activated – from Activity Bags to digital narratives, from tactile paths to multisensory experiences – respond to different needs by enhancing a plurality of languages and perceptions. A model of the museum emerges as a relational space, where accessibility becomes a continuous and shared practice of cultural citizenship.